Publicaciones representativas

P-Rex1 Signaling Hub in Lower Grade Glioma Patients, Found by In Silico Data Mining, Correlates With Reduced Survival and Augmented Immune Tumor Microenvironment.
Beltrán-Navarro YM, Reyes-Cruz G, Vázquez-Prado J.
Front Oncol. 2022 Jul 7;12:922025. doi: 10.3389/fonc.2022.922025.

Gβγ mediates activation of Rho guanine nucleotide exchange factor ARHGEF17 that promotes metastatic lung cancer progression.
García-Jiménez I, Cervantes-Villagrana RD, Del-Río-Robles JE, Castillo-Kauil A, Beltrán-Navarro YM, García-Román J, Reyes-Cruz G, Vázquez-Prado J.
J Biol Chem. 2022 Jan;298(1):101440. doi: 10.1016/j.jbc.2021.101440.

Gαs directly drives PDZ-RhoGEF signaling to Cdc42.
Castillo-Kauil A, García-Jiménez I, Cervantes-Villagrana RD, Adame-García SR, Beltrán-Navarro YM, Gutkind JS, Reyes-Cruz G, Vázquez-Prado J.
J Biol Chem. 2020 Dec 11;295(50):16920-16928. doi: 10.1074/jbc.AC120.015204.

Gβγ recruits and activates P-Rex1 via two independent binding interfaces.
Cervantes-Villagrana RD, Beltrán-Navarro YM, García-Jiménez I, Adame-García SR, Olguín-Olguín A, Reyes-Cruz G, Vázquez-Prado J.
Biochem Biophys Res Commun. 2021 Feb 5;539:20-27. doi: 10.1016/j.bbrc.2020.12.089.

Endothelial cell sprouting driven by RhoJ directly activated by a membrane-anchored Intersectin 1 (ITSN1) RhoGEF module.
Color-Aparicio VM, Cervantes-Villagrana RD, García-Jiménez I, Beltrán-Navarro YM, Castillo-Kauil A, Escobar-Islas E, Reyes-Cruz G, Vázquez-Prado J.
Biochem Biophys Res Commun. 2020 Mar 26;524(1):109-116. doi: 10.1016/j.bbrc.2020.01.068.

Protumoral bone marrow-derived cells migrate via Gβγ-dependent signaling pathways and exhibit a complex repertoire of RhoGEFs.
Cervantes-Villagrana RD, Color-Aparicio VM, Reyes-Cruz G, Vázquez-Prado J.
J Cell Commun Signal. 2019 Jan 5. doi: 10.1007/s12079-018-00502-6.

cAMP-dependent activation of the Rac guanine exchange factor P-REX1 by type I protein kinase A (PKA) regulatory subunits.
Adame-García SR, Cervantes-Villagrana RD, Orduña-Castillo LB, Del Rio JC, Gutkind JS, Reyes-Cruz G, Taylor SS, Vázquez-Prado J.
J Biol Chem. 2018 Dec 10. pii: jbc.RA118.006691. doi: 10.1074/jbc.RA118.006691.

Gβγ signaling to the chemotactic effector P-REX1 and mammalian cell migration is directly regulated by Gαq and Gα13 proteins.
Cervantes-Villagrana RD, Adame-García SR, García-Jimenez I, Color-Aparicio VM, Beltran-Navarro YM, König GM, Kostenis E, Reyes-Cruz G, Gutkind JS, Vázquez-Prado J.
J Biol Chem. 2018 Nov 16. pii: jbc.RA118.006254. doi: 10.1074/jbc.RA118.006254.

Cell adhesion controlled by adhesion G protein-coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising intersectins and Elmo-Dock.
Hernández-Vásquez MN, Adame-García SR, Hamoud N, Chidiac R, Reyes-Cruz G, Gratton JP, Côté JF, Vázquez-Prado J.
J Biol Chem. 2017 Jul 21;292(29):12178-12191. doi: 10.1074/jbc.M117.780304.

Gβγ Pathways in Cell Polarity and Migration Linked to Oncogenic GPCR Signaling: Potential Relevance in Tumor Microenvironment.
Vázquez-Prado J, Bracho-Valdés I, Cervantes-Villagrana RD, Reyes-Cruz G.
Mol Pharmacol. 2016 Nov;90(5):573-586. Epub 2016 Sep 16. Review.

Protein Kinase A (PKA) Type I Interacts with P-Rex1, a Rac Guanine Nucleotide Exchange Factor: EFFECT ON PKA LOCALIZATION AND P-Rex1 SIGNALING.

Chávez-Vargas L, Adame-García SR, Cervantes-Villagrana RD, Castillo-Kauil A, Bruystens JG, Fukuhara S, Taylor SS, Mochizuki N, Reyes-Cruz G, Vázquez-Prado J.

J Biol Chem. 2016 Mar 18;291(12):6182-99. doi: 10.1074/jbc.M115.712216. Epub 2016 Jan 21.

Endothelial RhoGEFs: A systematic analysis of their expression profiles in VEGF-stimulated and tumor endothelial cells. Hernández-García R, Iruela-Arispe ML, Reyes-Cruz G, Vázquez-Prado J. Vascul Pharmacol. 2015 Oct 17. pii: S1537-1891(15)00220-7. doi: 10.1016/j.vph.2015.10.003.

Gβγ interacts with mTOR and promotes its activation. Robles-Molina E, Dionisio-Vicuña M, Guzmán-Hernández ML, Reyes-Cruz G, Vázquez-Prado J. Biochem Biophys Res Commun. 2014 Jan 22. pii: S0006-291X(14)00068-0. doi: 10.1016/j.bbrc.2014.01.044

The emerging mutational landscape of G proteins and G-protein-coupled receptors in cancer. O'Hayre M, Vázquez-Prado J, Kufareva I, Stawiski EW, Handel TM, Seshagiri S, Gutkind JS. Nat Rev Cancer. 2013 Jun;13(6):412-24

Regulation of mTORC1 complex assembly and signaling by GRp58/ERp57. Ramírez-Rangel I, Bracho-Valdés I, Vázquez-Macías A, Carretero-Ortega J, Reyes-Cruz G, Vázquez-Prado J. Mol Cell Biol. 2011

Sphingosine-1-phosphate receptor S1P1 is regulated by direct interactions with P-Rex1, a Rac guanine nucleotide exchange factor. Ledezma-Sánchez BA, García-Regalado A, Guzmán-Hernández ML, Vázquez-Prado J. Biochem Biophys Res Commun. 2010 Jan 22;391(4):1647-52.

Phosphatidylinositol 3,4,5-triphosphate-dependent Rac exchanger 1 (P-Rex-1), a guanine nucleotide exchange factor for Rac, mediates angiogenic responses to stromal cell-derived factor-1/chemokine stromal cell derived factor-1 (SDF-1/CXCL-12) linked to Rac activation, endothelial cell migration, and in vitro angiogenesis. Carretero-Ortega J, Walsh CT, Hernández-García R, Reyes-Cruz G, Brown JH, Vázquez-Prado J. Mol Pharmacol. 2010 Mar;77(3):435-42.

Differential inhibitor of Gbetagamma signaling to AKT and ERK derived from phosducin-like protein: effect on sphingosine 1-phosphate-induced endothelial cell migration and in vitro angiogenesis. Guzmán-Hernández ML, Vázquez-Macías A, Carretero-Ortega J, Hernández-García R, García-Regalado A, Hernández-Negrete I, Reyes-Cruz G, Gutkind JS, Vázquez-Prado J. J Biol Chem. 2009 Jul 3;284(27):18334-46.

G protein-coupled receptor-promoted trafficking of Gbeta1gamma2 leads to AKT activation at endosomes via a mechanism mediated by Gbeta1gamma2-Rab11a interaction. García-Regalado A, Guzmán-Hernández ML, Ramírez-Rangel I, Robles-Molina E, Balla T, Vázquez-Prado J, Reyes-Cruz G. Mol Biol Cell. 2008 Oct;19(10):4188-200.

P-Rex1 links mammalian target of rapamycin signaling to Rac activation and cell migration. Hernández-Negrete I, Carretero-Ortega J, Rosenfeldt H, Hernández-García R, Calderón-Salinas JV, Reyes-Cruz G, Gutkind JS, Vázquez-Prado J. J Biol Chem. 2007 Aug 10;282(32):23708-15.

Modular architecture and novel protein-protein interactions regulating the RGS-containing Rho guanine nucleotide exchange factors. Vázquez-Prado J, Basile J, Gutkind JS. Methods Enzymol. 2004;390:259-85.

Chimeric G alpha i2/G alpha 13 proteins reveal the structural requirements for the binding and activation of the RGS-like (RGL)-containing Rho guanine nucleotide exchange factors (GEFs) by G alpha 13. Vázquez-Prado J, Miyazaki H, Castellone MD, Teramoto H, Gutkind JS. J Biol Chem. 2004 Dec 24;279(52):54283-90.

P-REX2, a novel PI-3-kinase sensitive Rac exchange factor. Rosenfeldt H, Vázquez-Prado J, Gutkind JS. FEBS Lett. 2004 Aug 13;572(1-3):167-71.

Direct interaction of p21-activated kinase 4 with PDZ-RhoGEF, a G protein-linked Rho guanine exchange factor. Barac A, Basile J, Vázquez-Prado J, Gao Y, Zheng Y, Gutkind JS. J Biol Chem. 2004 Feb 13;279(7):6182-9.

Plexin B regulates Rho through the guanine nucleotide exchange factors leukemia-associated Rho GEF (LARG) and PDZ-RhoGEF. Perrot V, Vazquez-Prado J, Gutkind JS. J Biol Chem. 2002 Nov 8;277(45):43115-20.

Potent activation of RhoA by Galpha q and Gq-coupled receptors. Chikumi H, Vázquez-Prado J, Servitja JM, Miyazaki H, Gutkind JS. J Biol Chem. 2002 Jul 26;277(30):27130-4

Activation of endothelin ETA receptors induces phosphorylation of alpha1b-adrenoreceptors in Rat-1 fibroblasts. Vázquez-Prado J, Medina LC, García-Sáinz JA. J Biol Chem. 1997 Oct 24;272(43):27330-7.

Effect of phorbol myristate acetate on alpha 1-adrenergic action in cells expressing recombinant alpha 1-adrenoceptor subtypes. Vázquez-Prado J, García-Sáinz JA. Mol Pharmacol. 1996 Jul;50(1):17-22.